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Bachem fluorogenic substrate ac-devd-afc
Fluorogenic Substrate Ac Devd Afc, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+fluorogenic+substrate/fluorogenic+substrate+ac+devd+afc/pm30052510-155-9-12
Average 90 stars, based on 1 article reviews
fluorogenic substrate ac-devd-afc - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Activity Assay:

Article Title: MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis
Article Snippet: In all, 30 μ g of protein of untreated and treated cells in the presence or absence of inhibitors were used to determine caspase activity by measuring the cleavage of fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin (Ac-DEVD-AFC) (Bachem).

Article Title: Differential responsiveness of human hepatoma cells versus normal hepatocytes to TRAIL in combination with either histone deacetylase inhibitors or conventional cytostatics.
Article Snippet: Caspase-3 activity was measured using the synthetic fluorogenic substrate Ac-DEVD-AFC (Bachem, Weil am Rhein, Germany).

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Size and morphology of toxic oligomers of amyloidogenic proteins: a case study of human stefin B.
Article Snippet: Amyloid-induced toxicity is a well-known phenomenon but the molecular background remains unclear.. One hypothesis relates toxicity to amyloid–membrane interactions, predicting that amyloid oligomers make pores into membranes.. Therefore, the toxicity and membrane interaction of prefibrillar aggregates and individual oligomers of a non-pathological yet highly amyloidogenic protein human stefin B (cystatin B) was examined.

Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

FACS:

Article Title: MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis
Article Snippet: In all, 30 μ g of protein of untreated and treated cells in the presence or absence of inhibitors were used to determine caspase activity by measuring the cleavage of fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin (Ac-DEVD-AFC) (Bachem).

Article Title: Differential responsiveness of human hepatoma cells versus normal hepatocytes to TRAIL in combination with either histone deacetylase inhibitors or conventional cytostatics.
Article Snippet: Caspase-3 activity was measured using the synthetic fluorogenic substrate Ac-DEVD-AFC (Bachem, Weil am Rhein, Germany).

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Size and morphology of toxic oligomers of amyloidogenic proteins: a case study of human stefin B.
Article Snippet: Amyloid-induced toxicity is a well-known phenomenon but the molecular background remains unclear.. One hypothesis relates toxicity to amyloid–membrane interactions, predicting that amyloid oligomers make pores into membranes.. Therefore, the toxicity and membrane interaction of prefibrillar aggregates and individual oligomers of a non-pathological yet highly amyloidogenic protein human stefin B (cystatin B) was examined.

Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Plant Extract:

Article Title: MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis
Article Snippet: In all, 30 μ g of protein of untreated and treated cells in the presence or absence of inhibitors were used to determine caspase activity by measuring the cleavage of fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin (Ac-DEVD-AFC) (Bachem).

Article Title: Differential responsiveness of human hepatoma cells versus normal hepatocytes to TRAIL in combination with either histone deacetylase inhibitors or conventional cytostatics.
Article Snippet: Caspase-3 activity was measured using the synthetic fluorogenic substrate Ac-DEVD-AFC (Bachem, Weil am Rhein, Germany).

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Size and morphology of toxic oligomers of amyloidogenic proteins: a case study of human stefin B.
Article Snippet: Amyloid-induced toxicity is a well-known phenomenon but the molecular background remains unclear.. One hypothesis relates toxicity to amyloid–membrane interactions, predicting that amyloid oligomers make pores into membranes.. Therefore, the toxicity and membrane interaction of prefibrillar aggregates and individual oligomers of a non-pathological yet highly amyloidogenic protein human stefin B (cystatin B) was examined.

Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Protein Concentration:

Article Title: MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis
Article Snippet: In all, 30 μ g of protein of untreated and treated cells in the presence or absence of inhibitors were used to determine caspase activity by measuring the cleavage of fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin (Ac-DEVD-AFC) (Bachem).

Article Title: Differential responsiveness of human hepatoma cells versus normal hepatocytes to TRAIL in combination with either histone deacetylase inhibitors or conventional cytostatics.
Article Snippet: Caspase-3 activity was measured using the synthetic fluorogenic substrate Ac-DEVD-AFC (Bachem, Weil am Rhein, Germany).

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Size and morphology of toxic oligomers of amyloidogenic proteins: a case study of human stefin B.
Article Snippet: Amyloid-induced toxicity is a well-known phenomenon but the molecular background remains unclear.. One hypothesis relates toxicity to amyloid–membrane interactions, predicting that amyloid oligomers make pores into membranes.. Therefore, the toxicity and membrane interaction of prefibrillar aggregates and individual oligomers of a non-pathological yet highly amyloidogenic protein human stefin B (cystatin B) was examined.

Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Incubation:

Article Title: MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis
Article Snippet: In all, 30 μ g of protein of untreated and treated cells in the presence or absence of inhibitors were used to determine caspase activity by measuring the cleavage of fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin (Ac-DEVD-AFC) (Bachem).

Article Title: Differential responsiveness of human hepatoma cells versus normal hepatocytes to TRAIL in combination with either histone deacetylase inhibitors or conventional cytostatics.
Article Snippet: Caspase-3 activity was measured using the synthetic fluorogenic substrate Ac-DEVD-AFC (Bachem, Weil am Rhein, Germany).

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Size and morphology of toxic oligomers of amyloidogenic proteins: a case study of human stefin B.
Article Snippet: Amyloid-induced toxicity is a well-known phenomenon but the molecular background remains unclear.. One hypothesis relates toxicity to amyloid–membrane interactions, predicting that amyloid oligomers make pores into membranes.. Therefore, the toxicity and membrane interaction of prefibrillar aggregates and individual oligomers of a non-pathological yet highly amyloidogenic protein human stefin B (cystatin B) was examined.

Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Concentration Assay:

Article Title: MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis
Article Snippet: In all, 30 μ g of protein of untreated and treated cells in the presence or absence of inhibitors were used to determine caspase activity by measuring the cleavage of fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin (Ac-DEVD-AFC) (Bachem).

Article Title: Differential responsiveness of human hepatoma cells versus normal hepatocytes to TRAIL in combination with either histone deacetylase inhibitors or conventional cytostatics.
Article Snippet: Caspase-3 activity was measured using the synthetic fluorogenic substrate Ac-DEVD-AFC (Bachem, Weil am Rhein, Germany).

Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

Article Title: Size and morphology of toxic oligomers of amyloidogenic proteins: a case study of human stefin B.
Article Snippet: Amyloid-induced toxicity is a well-known phenomenon but the molecular background remains unclear.. One hypothesis relates toxicity to amyloid–membrane interactions, predicting that amyloid oligomers make pores into membranes.. Therefore, the toxicity and membrane interaction of prefibrillar aggregates and individual oligomers of a non-pathological yet highly amyloidogenic protein human stefin B (cystatin B) was examined.

Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.



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